Nepal Medical College

1. Triple Sugar Iron test .Principle:     Triple sugar iron agar test is used to determine whether gram negative bacilli utilize glucose and lactose or sucrose fermentatively and produce hydrogen sulfide (H 2 S).  It contains 10 parts of lactose: 10 parts of sucrose: 1 part of glucose and peptone.  Phenol red and ferrous sulphate serves as an indicator for acidification of medium and H 2 S production respectively. Glucose is utilized first by a fermentative organism and the entire medium becomes acidic (yellow) in 8 to 12 hours. Butt remains acidic even after 18 to 24 hours incubation peroid because of the presence of organic acids resulting from the fermentation of glucose under  anaerobic  conditions in the butt of the tube.The slant reverts to  alkaline  state that is indicated by red color as the fermentation products gets oxidised to  carbon dioxide  (CO 2 ) and water (H 2 O) and peptone in  aerobic  condition the...

1. WIDAL test Kit and Card - For diagnosis of salmonella typhi and paratyphi A and B infection i.e. enteric fever - Principle: Slide Agglutination test - Antigens used: O antigen (somatic antigen) of Salmonella typhi , H antigen ( flagellar antigen) of Salmonella typhi, AH and BH ( Flagellar antigen of Salmonella paratyphi A and B) 2. BHI broth and Selenite F. broth BHI broth: -Brain Heart Infusion Broth                    - Enrichment media                   - Used for blood culture and body fluid like peritoneal and pleural fluid culture                  - Blood: broth ratio = 1:10 Selenite F broth : selective and enrichment media for Salmonella and Shigella                           : F stands for feces                 ...